Abstract

Hypoxia is a major component of ischemic stroke. The prevalence of ischemic stroke is expected to increase as the global population ages and risk factors, like obesity, are on the rise. Nutrition is a modifiable risk factor for ischemic stroke. Increased dietary intake of folic acid (FA) has become an increasing problem in the U.S and other countries as more people are consuming at or above the recommended daily amount of FA. The impact of too much dietary FA on hypoxia is not well understood. This study aimed to investigate how increased dietary levels of FA impact hypoxia outcomes using Drosophila melanogaster as a model. Adult female and male w1118 Drosophila melanogaster flies were placed on control (CD) and 100 µM folic acid (FAD) diets. At 5 to 6 days old progeny were exposed to hypoxia for two hours prior to returning to normoxic conditions to model reperfusion. We observed escape behavior in hypoxia larvae, confirming exposure to hypoxia Using liquid chromatography-tandem mass spectrometry, elevated FA levels were observed in FAD compared to controls. We report increased acute hypoxia-induced mortality in FAD flies. Furthermore, FAD flies were not motivated to climb after hypoxia. Under normoxic conditions FAD flies had a higher velocity when descending during a climb. There was no impact of FA on neurodegeneration in brain tissue. The data collected from this study suggests that hypoxia has a negative effect on survival and climbing behavior in a fly model. Together these data suggest that increase dietary intake of FA can have negative health outcomes after hypoxia.